Highly dynamically evolved intermediate-age open clusters

We present a comprehensive UBVRI and Washington CT1T2 photometric analysis of seven catalogued open clusters, namely: Ruprecht 3, 9, 37, 74, 150, ESO 324-15 and 436-2. The multiband photometric data sets in combination with 2MASS photometry and Gaia astrometry for the brighter stars were used to estimate their structural parameters and fundamental astrophysical properties.We found that Ruprecht 3 and ESO 436-2 do not show self-consistent evidence of being physical systems. The remained studied objects are open clusters of intermediate age (9.0 ≤ log(t yr-1) ≤ 9.6), of relatively small size (rcls ~ 0.4-1.3 pc) and placed between 0.6 and 2.9 kpc from the Sun.We analysed the relationships between core, half-mass, tidal and Jacoby radii as well as half-mass relaxation times to conclude that the studied clusters are in an evolved dynamical stage. The total cluster masses obtained by summing those of the observed cluster stars resulted to be ~10-15 per cent of the masses of open clusters of similar age located closer than 2 kpc from the Sun. We found that cluster stars occupy volumes as large as those for tidally filled clusters.Fil: Piatti, Andres Eduardo. Universidad Nacional de Córdoba. Observatorio Astronómico de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Dias, Wilton S.. Universidade Federal de Itajuba; BrasilFil: Sampedro, Laura M.. Instituto de Astrofísica de Andalucía; España. Universidade de Sao Paulo; Brasi

Forrest B. Weinberg - A Tribute

In Memorium: Forrest B. Weinber

Coping with Change: The Lawyer\u27s Role

The following articles are the result of an experimental course entitled Current Problems of Small Business offered at Cleveland-Marshall College of Law. Primarily for third-year students, the course was designed to provide a practical learning experience in seminar format. The course focused on business issues, but also taught lawyering skills such as interviewing, counseling, negotiating and drafting. Thus, the students were provided a pragmatic learning experience that can be applied to legal practice

Alien Registration- Wilton, Austin S. (Saint John Plantation, Aroostook County)

https://digitalmaine.com/alien_docs/32483/thumbnail.jp

Forrest B. Weinberg - A Tribute

In Memorium: Forrest B. Weinber

The Changing Role of the Attorney with Respect to the Corporation

Supreme Court Justice Potter Stewart once stated that the propriety of a lawyer serving as a member of the Board of Directors of his corporate client remains, even today, a vexing problem of professional responsibility.” Historically, accountants have been assumed, as well as required, to be independent of any enterprise in which they express an opinion regarding the enterprise\u27s financial statements. Independence had been interpreted to mean that accountants may not serve on the board of directors or invest in any enterprise which they, or their firm, audit, or for whom either expresses an opinion on the enterprise\u27s financial statements. By contrast, attorneys have been counsel to an enterprise, have served as officers and directors, and have invested in the enterprise. The purposes of this article are to: (1) compare and contrast the difference between the accounting and the legal profession\u27s self-regulation of board membership; (2) analyze the trend towards requiring more independence of attorneys as it relates to simultaneously providing legal advice to a client and serving on the client\u27s board of directors; and (3) predict the future trends regarding attorneys serving as directors of clients

Analysis of pathogenic pseudoexons reveals novel mechanisms driving cryptic splicing

Understanding pre-mRNA splicing is crucial to accurately diagnosing and treating genetic diseases. However, mutations that alter splicing can exert highly diverse effects. Of all the known types of splicing mutations, perhaps the rarest and most difficult to predict are those that activate pseudoexons, sometimes also called cryptic exons. Unlike other splicing mutations that either destroy or redirect existing splice events, pseudoexon mutations appear to create entirely new exons within introns. Since exon definition in vertebrates requires coordinated arrangements of numerous RNA motifs, one might expect that pseudoexons would only arise when rearrangements of intronic DNA create novel exons by chance. Surprisingly, although such mutations do occur, a far more common cause of pseudoexons is deep-intronic single nucleotide variants, raising the question of why these latent exon-like tracts near the mutation sites have not already been purged from the genome by the evolutionary advantage of more efficient splicing. Possible answers may lie in deep intronic splicing processes such as recursive splicing or poison exon splicing. Because these processes utilize intronic motifs that benignly engage with the spliceosome, the regions involved may be more susceptible to exonization than other intronic regions would be. We speculated that a comprehensive study of reported pseudoexons might detect alignments with known deep intronic splice sites and could also permit the characterisation of novel pseudoexon categories. In this report, we present and analyse a catalogue of over 400 published pseudoexon splice events. In addition to confirming prior observations of the most common pseudoexon mutation types, the size of this catalogue also enabled us to suggest new categories for some of the rarer types of pseudoexon mutation. By comparing our catalogue against published datasets of non-canonical splice events, we also found that 15.7% of pseudoexons exhibit some splicing activity at one or both of their splice sites in non-mutant cells. Importantly, this included seven examples of experimentally confirmed recursive splice sites, confirming for the first time a long-suspected link between these two splicing phenomena. These findings have the potential to improve the fidelity of genetic diagnostics and reveal new targets for splice-modulating therapies